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Addgene inc balazsi g
Balazsi G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid/pDN-D2irTN2AG5kwh+(Plasmid+%23128253)/pm42926929-191-9-11
Average 91 stars, based on 4 article reviews
balazsi g - by Bioz Stars, 2026-11
91/100 stars

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Expressing:

Article Title: Local gene editing of fibroblasts in tumors reveals a new cancer-associated fibroblast state
Article Snippet: .. To generate NIH/3T3 cells expressing Cas9 (NIH/3T3.Cas9.EGFP), NIH/3T3 cells were lentivirally transduced with the viral supernatant produced in Lenti-X 293T cells after transfection with the following plasmids: cargo plasmid containing Cas9 and EGFP (pL-CRISPR.EFS-GFP, #57818; Addgene), VSV-G envelope–containing plasmid (pMD2.G, #12259; Addgene), and envelope plasmid (pCMV-dR8.91, #OVT2971; Creative Biogene) at a molar ratio of 1:0.44:0.8, respectively, and added polyethylenimine (PEI), linear, MW 25000 (#23966; Polysciences) at a PEI-to-DNA ratio of 4-to-1. .. Two days after Lenti-X 293T transfection, viral supernatant was filtered through a 0.45-μm filter (Whatman) and added to NIH/3T3 cells with supplemented polybrene (#TR-1003-G; Sigma-Aldrich) at a final concentration of 8 μg/ml.

Transduction:

Article Title: Local gene editing of fibroblasts in tumors reveals a new cancer-associated fibroblast state
Article Snippet: .. To generate NIH/3T3 cells expressing Cas9 (NIH/3T3.Cas9.EGFP), NIH/3T3 cells were lentivirally transduced with the viral supernatant produced in Lenti-X 293T cells after transfection with the following plasmids: cargo plasmid containing Cas9 and EGFP (pL-CRISPR.EFS-GFP, #57818; Addgene), VSV-G envelope–containing plasmid (pMD2.G, #12259; Addgene), and envelope plasmid (pCMV-dR8.91, #OVT2971; Creative Biogene) at a molar ratio of 1:0.44:0.8, respectively, and added polyethylenimine (PEI), linear, MW 25000 (#23966; Polysciences) at a PEI-to-DNA ratio of 4-to-1. .. Two days after Lenti-X 293T transfection, viral supernatant was filtered through a 0.45-μm filter (Whatman) and added to NIH/3T3 cells with supplemented polybrene (#TR-1003-G; Sigma-Aldrich) at a final concentration of 8 μg/ml.

Produced:

Article Title: Local gene editing of fibroblasts in tumors reveals a new cancer-associated fibroblast state
Article Snippet: .. To generate NIH/3T3 cells expressing Cas9 (NIH/3T3.Cas9.EGFP), NIH/3T3 cells were lentivirally transduced with the viral supernatant produced in Lenti-X 293T cells after transfection with the following plasmids: cargo plasmid containing Cas9 and EGFP (pL-CRISPR.EFS-GFP, #57818; Addgene), VSV-G envelope–containing plasmid (pMD2.G, #12259; Addgene), and envelope plasmid (pCMV-dR8.91, #OVT2971; Creative Biogene) at a molar ratio of 1:0.44:0.8, respectively, and added polyethylenimine (PEI), linear, MW 25000 (#23966; Polysciences) at a PEI-to-DNA ratio of 4-to-1. .. Two days after Lenti-X 293T transfection, viral supernatant was filtered through a 0.45-μm filter (Whatman) and added to NIH/3T3 cells with supplemented polybrene (#TR-1003-G; Sigma-Aldrich) at a final concentration of 8 μg/ml.

Transfection:

Article Title: Local gene editing of fibroblasts in tumors reveals a new cancer-associated fibroblast state
Article Snippet: .. To generate NIH/3T3 cells expressing Cas9 (NIH/3T3.Cas9.EGFP), NIH/3T3 cells were lentivirally transduced with the viral supernatant produced in Lenti-X 293T cells after transfection with the following plasmids: cargo plasmid containing Cas9 and EGFP (pL-CRISPR.EFS-GFP, #57818; Addgene), VSV-G envelope–containing plasmid (pMD2.G, #12259; Addgene), and envelope plasmid (pCMV-dR8.91, #OVT2971; Creative Biogene) at a molar ratio of 1:0.44:0.8, respectively, and added polyethylenimine (PEI), linear, MW 25000 (#23966; Polysciences) at a PEI-to-DNA ratio of 4-to-1. .. Two days after Lenti-X 293T transfection, viral supernatant was filtered through a 0.45-μm filter (Whatman) and added to NIH/3T3 cells with supplemented polybrene (#TR-1003-G; Sigma-Aldrich) at a final concentration of 8 μg/ml.

Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing.
Article Snippet: .. On day 1, cells were transfected with jetPRIME transfection reagent in a transfection mixture containing 2 μg of plasmid, 0.2 μg of Sleeping Beauty transposase plasmid (Addgene 34879), 4 μl of jetPRIME reagent and 200 μl of jetPRIME buffer according to the manufacturer’s protocol. ..

Article Title: Adult-onset deficiency of β-klotho in the liver reveals a novel role in lipid homeostasis.
Article Snippet: 402 403 4.2 Adeno-associated virus generation 404 Expression cassettes encoding sgRNA sequences targeting exon 1 of Klb (sgRNA1: 405 TTACCGGCTTCTCCGGAGAC, sgRNA2: TAAAAAACAGTACGTGAGTC, sgRNA3: 406 TGCTCCGGTCCCAACGCCCC) and targeting both 1 and 2 (sgRNA1: TTGGAGACCGTGTCAAATAT; 407 sgRNA2: CATGCCAAGCAACAAGGTAA; sgRNA3: TAGTCGCCGTCCCCGTGGAT) were synthesized 408 Jo ur na l P re -p ro of 14 and cloned into an scAAV2 backbone vector using Kpnl and Notl (New England Biolabs), after which 409 sequence verification was performed. .. HEK293T cells with adenovirus early region 1 encoded within 410 their genome were transfected with the vector, a pAd-deltaF6 helper plasmid (112867, Addgene; a gift 411 from James M. Wilson) and a pAAV2/8 Rep/Cap-encoding plasmid (112864, Addgene; a gift from James 412 M. Wilson) using polyethylenimine (23966-2, Polysciences Inc). .. An iodixanol gradient was used to 413 isolate the virus particles produced by the cells, followed by spin filter centrifugation with a 100 kDa cut-414 off (UFC910024, Merck) to concentrate the particles.

Plasmid Preparation:

Article Title: Local gene editing of fibroblasts in tumors reveals a new cancer-associated fibroblast state
Article Snippet: .. To generate NIH/3T3 cells expressing Cas9 (NIH/3T3.Cas9.EGFP), NIH/3T3 cells were lentivirally transduced with the viral supernatant produced in Lenti-X 293T cells after transfection with the following plasmids: cargo plasmid containing Cas9 and EGFP (pL-CRISPR.EFS-GFP, #57818; Addgene), VSV-G envelope–containing plasmid (pMD2.G, #12259; Addgene), and envelope plasmid (pCMV-dR8.91, #OVT2971; Creative Biogene) at a molar ratio of 1:0.44:0.8, respectively, and added polyethylenimine (PEI), linear, MW 25000 (#23966; Polysciences) at a PEI-to-DNA ratio of 4-to-1. .. Two days after Lenti-X 293T transfection, viral supernatant was filtered through a 0.45-μm filter (Whatman) and added to NIH/3T3 cells with supplemented polybrene (#TR-1003-G; Sigma-Aldrich) at a final concentration of 8 μg/ml.

Article Title: New selective and allosteric FLT3 inhibitors show efficacy against resistant acute myeloid leukemia cells
Article Snippet: .. psPAX2 , Addgene , Plasmid #12260. .. pMD2.G , Addgene , Plasmid #12259.

Article Title: Engineered human Argonaute 2 for targeted C-to-U RNA base editing.
Article Snippet: .. On day 1, cells were transfected with jetPRIME transfection reagent in a transfection mixture containing 2 μg of plasmid, 0.2 μg of Sleeping Beauty transposase plasmid (Addgene 34879), 4 μl of jetPRIME reagent and 200 μl of jetPRIME buffer according to the manufacturer’s protocol. ..

Article Title: Adult-onset deficiency of β-klotho in the liver reveals a novel role in lipid homeostasis.
Article Snippet: 402 403 4.2 Adeno-associated virus generation 404 Expression cassettes encoding sgRNA sequences targeting exon 1 of Klb (sgRNA1: 405 TTACCGGCTTCTCCGGAGAC, sgRNA2: TAAAAAACAGTACGTGAGTC, sgRNA3: 406 TGCTCCGGTCCCAACGCCCC) and targeting both 1 and 2 (sgRNA1: TTGGAGACCGTGTCAAATAT; 407 sgRNA2: CATGCCAAGCAACAAGGTAA; sgRNA3: TAGTCGCCGTCCCCGTGGAT) were synthesized 408 Jo ur na l P re -p ro of 14 and cloned into an scAAV2 backbone vector using Kpnl and Notl (New England Biolabs), after which 409 sequence verification was performed. .. HEK293T cells with adenovirus early region 1 encoded within 410 their genome were transfected with the vector, a pAd-deltaF6 helper plasmid (112867, Addgene; a gift 411 from James M. Wilson) and a pAAV2/8 Rep/Cap-encoding plasmid (112864, Addgene; a gift from James 412 M. Wilson) using polyethylenimine (23966-2, Polysciences Inc). .. An iodixanol gradient was used to 413 isolate the virus particles produced by the cells, followed by spin filter centrifugation with a 100 kDa cut-414 off (UFC910024, Merck) to concentrate the particles.

other:

Article Title: Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons.
Article Snippet: The Laconic construct used for neuronal transduction was derived from the same plasmid (44238, Addgene) used for astrocyte transduction.

Article Title: Suppression of Tau-tubulin kinase 1 mitigates tau phosphorylation, and Aβ mediated neurotoxicity via altered kinase network.
Article Snippet: The target shRNA sequences were previously reported (7) and cloned into the plasmid which included pAAV-U6-gRNA-hSyn-mCherry sequence (#87916, Addgene).

Article Title: Engineering of the High-Precision Cas12m Dual Base Editor-Loaded Virus-like Particles for A-to-G and C-to-T Conversions
Article Snippet: psgRNA-Cas12m was obtained based on a donor plasmid (#122089, Addgene) by replacing the original sequence with the target sequence (Supplementary Figure S5A) via PciI and KpnI sites.

Article Title: A splicing factor-cohesin association links RNA processing to cohesin-dependent genome folding.
Article Snippet: The TurboID sequence was amplified by PCR from the plasmid (Addgene, # 107169), and the human MAU2 coding sequence was obtained by PCR from a cDNA library using the primers listed in Table S5.



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