Review

addgene 43802 (Addgene inc)

Addgene inc is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Addgene inc addgene 43802
Addgene 43802, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-10-10
Average 93 stars, based on 126 article reviews

addgene 43802 - by Bioz Stars, 2026-09

93/100 stars

Images

Expressing:

Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems
Article Snippet: pMAL expression vector (MBP-fusion; Amp R ) , Addgene , Plasmid# 161783. .. MBP- mCherry expression plasmid (Amp R ) , Addgene , Plasmid# 29747. ..

Plasmid Preparation:

Article Title: Ubiquitin ligase CHFR impairs Tie2 signaling via K 48 -linked ubiquitylation and degradation of Akt1 in endothelial cells
Article Snippet: Scrambled-siRNA (Sc-siRNA) and human ( h )-specific siRNA1 against CHFR (A pool of 3 target-specific siRNAs, sense: 5′-CUCUGUGGCAAGUGAUGAAtt-3′; sense: 5′-GAAGAAGAUGUGCAAAGUAtt; sense: 5′-GGUAUAGUAUGGUAUUUGAtt-3′) were obtained from Santa Cruz Biotechnology. pEGFP-C2 plasmid (Addgene catalog #61853) encoding wild-type human CHFR (WT h -CHFR), h CHFR mutant lacking forkhead-associated domain (ΔFHA-CHFR), h CHFR mutant lacking RING finger domain (ΔRF-CHFR), mutant lacking cysteine-rich domain (ΔCR-CHFR), and mutant lacking poly-ADP ribose binding zinc-finger domain (ΔPBZ-CHFR) were custom prepared by Genscript (Piscataway, NJ). pmcherry-C1 plasmid (Addgene catalog #86631) encoding wild-type human Akt1 (WT h -Akt1), phopsho-defective h Akt1 (T308A and S473A) mutant and ubiquitylation-defective h Akt1 (K30R, K39R, K39R+154R and K30R+K39R+K154R+K268R) mutants were custom prepared by Genscript (Piscataway, NJ). .. HA-tagged WT-ubiquitin-expressing plasmid (catalog #17608) and HA-tagged K48- or K63-ubiquitin-expressing plasmids (where all the lysine residues were mutated to Arginine except K48 or K63) were from Addgene. ..

other:

Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems
Article Snippet: pGRG36 (Tn7 delivery vector; Amp R ) , Addgene , Plasmid #16666.

Article Title: New selective and allosteric FLT3 inhibitors show efficacy against resistant acute myeloid leukemia cells
Article Snippet: psPAX2 , Addgene , Plasmid #12260.

Article Title: KELPE: knock-in exchangeable dual landing pad embryonic stem cells enable efficient screening of synthetic gene circuits
Article Snippet: An equivalent plasmid is available on Addgene (plasmid # 62658).

Article Title: New selective and allosteric FLT3 inhibitors show efficacy against resistant acute myeloid leukemia cells
Article Snippet: pMD2.G , Addgene , Plasmid #12259.

Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs
Article Snippet: PX458 , Addgene , Plasmid #48138.

Construct:

Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein
Article Snippet: .. To construct gRNA expression plasmids, duplex oligonucleotides encoding hAAVS1 or pROSA26 gRNA ( ) were ligated into the pX330 all-in-one plasmid (#42230, Addgene), which was restricted with BbsI (#R3539L, NEB). .. The ligation product was transformed into One ShotTM Stbl3TM Chemically Competent E . coli (#C7373-03, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.